[Abstract] Objective: To study the effect of thrombin-like and fibrinolytic enzymes isolated and purified from the same Agkistrodon acutus venom on the blood coagulation system. Methods: The thrombin-like and fibrinolytic enzymes were isolated and purified from Agkistrodon acutus venom by DEAE-Se...
[Abstract] Objective: To study the effect of Agkistrodon acutus venom tumor suppressor component I (AAVC-I) on the proliferation inhibition and apoptosis of human lung cancer A549 cells. Methods: The inhibition rates of AAVC-I at different concentrations on A549 cells for 24h and 48h were measure...
[Abstract] Objective: To study the effect of Agkistrodon acutus venom tumor suppressor component I (AAVC-I) on the proliferation inhibition and apoptosis of human lung cancer A549 cells. Methods: The inhibition rates of AAVC-I at different concentrations on A549 cells for 24h and 48h were measure...
[Abstract] In this paper, the effects of the anticoagulant component of Agkistrodon acutus venom on thrombosis and thrombin time were studied. The results showed that this component could shorten thrombin time, and the thrombus formed after this component was coagulated. Soluble in 30% urea solut...
[Abstract] Snake venom contains a variety of metal proteins, which have different biological activities, such as anticoagulant factor (ACF), glycoside hydrolase (NADase), fibrinolytic component (FP) and haemorrhagic toxin. Studies on their biological activities, fluorescence spectra and structura...
[Abstract] [Objective] To prepare IgY antibody against the common motif of metalloproteinases from Agkistrodon acutus venom, and to study its effect on the activity of snake venom [Methods] By analyzing the common sequences of various metalloproteinases from Agkistrodon acutus venom, we designed ...
[Abstract] Objective To investigate the inhibitory effect of small molecular polypeptide fraction (K fraction) from Agkistrodon acutus venom on the proliferation of human ovarian cancer cell line A2780 and its mechanism. Methods MTT assay was used to detect the growth inhibition of K component on...
To study the effect of thrombin-like and fibrinolytic enzymes isolated and purified from the same Agkistrodon acutus venom on the blood coagulation system. Methods: The thrombin-like and fibrinolytic enzymes were isolated and purified from Agkistrodon acutus venom by DEAE-Sepharose CL-6B and Seph...
To discuss the pharmacological effects of Agkistrodon acutus venom. Methods: The latest research progress of chemical constituents, coagulation, antithrombotic effect, anti-tumor mechanism and other physiological activities of Agkistrodon acutus venom was reviewed. Results: The study shows that A...
The effect of the coagulating component of Agkistrodon acutus venom on thrombosis and thrombin time was studied. The results showed that the component could shorten the thrombin time and the thrombus formed after the component was coagulated. It can dissolve in 30% urea solution. The clotting tim...
[Abstract] Objective To isolate and purify anticoagulant protein from Agkistrodon acutus venom in southern Anhui and observe its inhibitory effect on human vascular endothelial cells. Methods Human vascular endothelial cells EC-304 were cultured in vitro. CCK-8 colorimetry was used to determine t...
[Abstract] Objective To isolate and purify anticoagulant protein from Agkistrodon acutus venom in southern Anhui and observe its inhibitory effect on human vascular endothelial cells. Methods Human vascular endothelial cells EC-304 were cultured in vitro. CCK-8 colorimetry was used to determine t...